See also how to create a reverse complement sequence.
Each nucleotide in a double stranded DNA molecule is paired with its Watson-Crick counterpart. This counterpart is called its complementary nucleotide.
Example:
Original sequence: ACGTATAGGCTGACACGTAGAGATGGATGACCATAG
Reverse sequence: GATACCAGTAGGTAGAGATGCACAGTCGGATATGCA
Complement sequence: TGCATATCCGACTGTGCATCTCTACCTACTGGTATC
Reverse complement: CTATGGTCATCCATCTCTACGTGTCAGCCTATACGT
List of all complement nucleotides:
Base | Name / Bases | Complementary Base |
---|---|---|
A | Adenine | T |
C | Cytidine | G |
G | Guanidine | C |
T | Thymidine | A |
Y | Pyrimidine (CT) | R |
R | Purine (AG) | Y |
S | Strong (GC) | S |
W | Weak (AT) | W |
K | Keto (TG) | M |
M | Amino (AC) | K |
B | not A (CGT) | V |
D | not C (AGT) | H |
H | not G (ACT) | D |
V | not T (ACG) | B |
N | Unknown (ACGT) | N |
See the Tutorial on how to create reverse complement sequence.